All-in-One Transcription MasterMix for PCR

TransScript II All-in-OneFirst-Strand cDNA Synthesis SuperMix for PCR   TransScript® II All-in-One First-Strand cDNA Synthesis SuperMix for PCR provides all the necessary components for cDNA synthesis from total RNA or mRNA. The supermix is provided at 5× concentration and used at 1× concentration by adding RNA and H2O. The resulting cDNA are suitable for regular PCR and not for qPCR.  

Cat. No.	AH321
Specification	AH321-01	50 rxns  (20 μl per reaction
Storage:	at -20°C for one year
Advantages	One-tube format for simple and fast setup by adding RNA template and water
	The optimal ratio of oligo(dT) primer to random primer(N9) and optimized SuperMix components to permit high-efficiency synthesis of long cDNA
	Reverse transcription for only 30 minutes
	High compatibility with PCR reagents
	cDNA fragment up to 15 kb
Applications	cDNA library construction, 3’ and 5’ RACE
	Multiple copy and low copy gene synthesis
	GC-rich or complex secondary structure RNA template

    Kit Contents 

Component	AH321-01
5×TransScript ® II All-in-One SuperMix for PCR	200 μl
RNase-free Water	1 ml

  First-Strand cDNA synthesis  1. Reaction Components

Component	Volume
Total RNA/mRNA	50 ng-5 μg/5-500 ng
5×TransScript® II All-in-One SuperMix for PCR	4 μl
RNase-free Water	to 20 μl

    Reaction Components

Component	Volume	Final Concentration
cDNA	2 μl	as required
Forward Primer (10 μM)	1 μl	0.2 μM
Reverse Primer (10 μM)	1 μl	0.2 μM
10×TransTaq® HiFi PCR SuperMix II	25 μl	1×
ddH2O	to 50 μl	-

    Packaging