Fast Mutagenesis System

  Fast Mutagenesis System  

Cat. No.	FM111
Specification	FM111-01: 5 rxns
	FM111-02: 20 rxns
Storage	DMT Chemically Competent Cells at -70oC for six months; others at -20oC for two years
Advantages	• Mutation sites on both primers to improve the mutation efficiency.
	• Partially overlapping primers for exponential DNA amplification.
	• TransStart®FastPfu DNA Polymerase has the extension rate of 4 kb/min and fidelity is 54 times higher than EasyTaq® DNA Polymerase.
	• Double digestions (in vitro and in vivo) of parental plasmids to enhance mutation efficiency

    Kit Contents

Component	FM111-01 (5 rxns)	FM111-02 (20 rxns)
TransStart® FastPfu DNA Polymerase (2.5 units/μl)	20 units	60 units
5× TransStart® FastPfu Buffer(with Mg2+)	100 μl	400 μl
10 mM dNTPs	15 μl	50 μl
50 mM MgSO4	50 μl	50 μl
DMT Enzyme (10 units/μl)	6 μl	22 μl
SControl Plasmid (5 ng/μl)	5 μl	25 μl
SControl Primers (100 ng/μl)	5 μl	25 μl
DMT Chemically Competent Cell	5×50 µl	20×50 µl

   Application Site-directed mutagenesis with plasmid ≤20 kb   CITATIONS Wang C, et al. 2013. Identification of BZR1-interacting proteins as potential components of the brassinosteroid signaling pathway in Arabidopsis through tandem affinity purification. Mol Cell Proteomics. IF=7.251. PMID: 24019147.   Cao X, et al. 2013. Mutation in AQP5, Encoding Aquaporin 5, Causes Palmoplantar Keratoderma Bothnia Type. J Invest Dermatol. IF=6.193. PMID: 23867895.   Zhang H F, et al. 2013. miR-200b suppresses invasiveness and modulates the cytoskeletal and adhesive machinery in esophageal squamous cell carcinoma cells via targeting Kindlin-2. Carcinogenesis. IF=5.635. PMID: 24064224.   Wang X, et al. 2013. Gestational Hypoxia Induces Sex-Differential Methylation of Crhr1 Linked to Anxiety-like Behavior. Mol Neurobiol. IF=5.471. PMID: 23529784.   Gong W, et al. 2013. Chronic Inhibition of Cyclic Guanosine Monophosphate-Specific Phosphodiesterase 5 Suppresses Endoplasmic Reticulum Stress in Heart Failure. Br J Pharmacol. IF=5.067. PMID: 24032459.   Zhang J, et al. 2013. Neuroglobin, a Novel Intracellular Hexa-Coordinated Globin, Functions as a Tumor Suppressor in Hepatocellular Carcinoma via Raf/MAPK/Erk. 83(5):1109-19. Mol Pharmacol. IF=4.411. PMID: 23478801.   Zhang H, et al. 2013. The regulation of the UCH-L1 gene by transcription factor NF-κB in podocytes. 25(7):1574-85. Cell Signal. IF=4.304. PMID: 23567262.   Zhang S, et al. 2013. Distinct post-transcriptional regulation of Igfbp1 gene by hypoxia in lowland mouse and Qinghai-Tibet plateau root vole Microtus oeconomus. 376(1-2):33-42. Mol Cell Endocrinol. IF=4.039. PMID: 23748030.    Li M, et al. 2013. Stability and iron oxidation properties of a novel homopolymeric plant ferritin from adzuki bean seeds: A comparative analysis with recombinant soybean seed H-1 chain ferritin. 1830(4):2946-53. Biochim Biophys Acta. IF=3.848. PMID: 23313843.   Meng D, et al. 2013. Identification and active site analysis of the 1-aminocyclopropane-1-carboxylic acid oxidase catalyzing the synthesis of ethylene in Agaricus bisporus. 1840(1):120-128. Biochim Biophys Acta. IF=3.848. PMID: 24016603.   Cheng S X, et al. 2013. FoxM1 Promotes Glioma Cells Progression by Up-Regulating Anxa1 Expression. 8(8):e72376. PLoS One. IF=3.73. PMID: 23991102.   Chu X, et al. 2013. Functional consequences of a novel point mutation in the CYP21A2 gene identified in a Chinese Han patient with nonclassic 21-hydroxylase deficiency. Clin Endocrinol. IF=3.396. PMID: 23927611.   Ni Z, et al. 2013. GmNFYA3, a target gene of miR169, is a positive regulator of plant tolerance to drought stress. 82(1-2):113-29. Plant Mol Biol. IF=3.518. PMID: 23483290.   Lin Z. et al. 2012. Exome sequencing reveals mutations in TRPV3 as a cause of Olmsted syndrome. 90(3):558-64. Am J Hum Genet. IF= 10.603.  PMID: 22405088.   Yu J. et al. 2011. PDlim2 selectively interacts with the PDZ binding motif of highly pathogenic avian H5N1 influenza A virus NS1. 6(5):e19511. PLoS One. IF= 4.092.  PMID: 21625420.   Sun HT. et al. 2013. FoxQ1 Promotes Glioma Cells Proliferation and Migration by Regulating NRXN3 Expression. 8(1):e55693. PLoS One. IF= 4.092.  PMID: 23383267.   Bi FC. et al. 2011. A conserved cysteine motif is critical for rice ceramide kinase activity and function. 6(3):e18079. PLoS One. IF= 4.092.  PMID: 21483860.   Yang J. et al. 2012. Enhancing production of bio-isoprene using hybrid MVA pathway and isoprene synthase in E. coli. 7(4):e33509. PLoS One. IF= 4.092.  PMID: 22558074.   Chen F. et al. 2012. Let-7b inhibits human cancer phenotype by targeting cytochrome P450 epoxygenase 2J2. 7(6):e39197. PLoS One. IF= 4.092.  PMID: 22761738.   Lyu ZX. et al. 2012. Direct observation of the uptake of outer membrane proteins by the periplasmic chaperone Skp. 7(9):e46068. PLoS One. IF= 4.092.  PMID: 23049938.   Xu S. et al. 2012. Maternal xNorrin, a canonical Wnt signaling agonist and TGF-β antagonist, controls early neuroectoderm specification in Xenopus. 10(3):e1001286. PLoS Biol. IF= 12.69.  PMID: 22448144.   Chang F. et al. 2012. AtPRK2 Promotes ROP1 Activation via RopGEFs in the Control of Polarized Pollen Tube Growth. Mol Plant. IF= 5.546.  PMID: 23024212.   Chu Y. et al. 2012. Identification and characterization of a highly conserved crenarchaeal protein lysine methyltransferase with broad substrate specificity. 194(24):6917-26. J Bacteriol. IF= 3.825.  PMID: 23086207.   Ouyang M. et al. 2011. LTD is a protein required for sorting light-harvesting chlorophyll-binding proteins to the chloroplast SRP pathway. 2:277. Nat Commun. IF= 7.396.  PMID: 21505433.                   Packaging