Erythromycin ELISA Test Kit

 

Product Description

REAGEN^™Erythromycin ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Erythromycin in meat(beff,chicken and pork),fish and shrip sampless. The unique features of the kit are:

l          High recovery (80-105%),rapid(less than 40 minutes) extraction methods for various samples.

l          High sensitivity (0.5ng/g or ppb)and low detection limit (2.5ppb)for fish and shrimp samples.

l          High reproducibility..

l          A quick ELISA assay(less than 2 hours regardless of number of samples).

Procedure Overview

The method is based on a competitive colorimetric ELISA assay. The drug of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target drug. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. The secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.

Kit Contents, Storage and Shelf Life

REAGEN^™Erythromycin ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C *. The shelf life is 12 months when the kit is properly stored.

*  If you are not planning to use the kit for over 1 month, store  Erythromycin     Standards, Erythromycin Antibody and  1X HRP Conjugate at -20°C or in a freezer.

Sensitivity (Detection Limit)

Sample Type	Detection  Limit (ppb)
Meat/Fish/shrip	2.5 ppb

Specificity (Cross-Reactivity)

Required Materials Not Provided With the Kit

l          Microtiter plate reader (450 nm)

l          Tissue Mixer (e.g. Omni TissueMaster Homogenizer)

l          Vortex mixer (e.g. Genie Vortex mixer from VWR)

l          10, 20, 100 and 1000 µL pipettes

l          Multi-channel pipette: 50-300 µL (Optional)

Warnings and Precautions

l          The standards contain Erythromycin. Handle with particular care.

l          Do not use the kit past the expiration date.

l          Do not intermix reagents from different kits or lots except for components with the same part No`s within  their  expiration  dates. ANTIBODIES  AND PLATES  ARE  KIT-AND LOT-SPECIFIC.  Make sure that the HRP Conjugate and Diluent are mixed in correct volumes.

l          Try to maintain a laboratory temperature of 20°–25°C (68°–77°F). Avoid running assays under or near air vents, as this may cause excessive cooling, heating and/or evaporation. Also, do not run assays  in  direct sunlight,  as this may cause excessive  heat and evaporation. Cold  bench tops should be avoided by placing several layers of paper towel or some other insulate material under the assay plates during incubation.

l          Make sure you are using only distilled or deionized water since water quality is very important.

l          When pipetting samples or reagents  into an empty microtiter plate, place the pipette tips in the lower corner of the well, making contact with the plastic.

l          Incubations of assay plates should be timed as precisely as possible. Be consistent when adding standards to the assay plate. Add your standards  first and then your samples.

l          Add standards  to plate only in the order from low concentration to high concentration, as this will minimize the risk of compromising the standard curve.

Always  refrigerate  plates  in  sealed  bags  with  a  desiccant  to  maintain  stability.  Prevent condensation from forming on plates by allowing them equilibrate to room temperature  (20 – 25°C /68 – 77°F) while in the packaging