100bp Plus II DNA Ladder

100bp Plus II DNA Ladder 100bp Plus II DNA Ladder is composed of fourteen linear double-stranded DNA bands. This ready-to-use product is premixed with 1× DNA loading buffer. Based on experiment requirement, 5 μl marker can be directly loaded for gel electrophoresis. It provides simple operation and produces clear image. For easy reference on agarose gels, the 500 bp band (100 ng/5 μl) is brighter than the other bands (50 ng/5 μl).  

Cat. No.	BM321
Specification	BM321-01	500 µl
	BM321-02	5×500 µl
Storage:	at 4oC for six months; at -20oC for two years
Concentration:	0.15 mg/ml
Advantages	Suitable for accurate determination of the size of DNA fragment within the range of 100 bp-5000 bp.
	Simultaneous determination of the size of enzyme-digested vector and insert fragments.
Composition	100 bp, 200 bp, 300 bp, 400 bp, 500 bp, 600 bp, 700 bp, 800 bp, 900 bp, 1,000 bp, 1500 bp, 2000 bp, 3000 bp, 5000 bp
Storage Buffer	10 mM Tris-HCl (pH 8.4), 10 mM EDTA, 0.02% bromophenol blue, 5% glycerol
Instructions for Use	Load 100bp Plus II DNA Ladder on the lane on a 2-3% agarose gel (load 1 μl marker for 1 mm lane, increase the loading volume for wider lane ).
	Suggested electrophoresis conditions: 2-3% agarose gel, voltage 4-10 v/cm.
	Stained with EB or GelStain (GS101), visualize the bands under exposure to ultraviolet light.
Notes	Completely thaw and mix well before use.
	Please change the electrophoresis buffer in time and use freshly-prepared gel to avoid adverse effect on the result.

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