Nitroimidazole ELISA Test Kit

Name: Nitroimidazole ELISA Test Kit
Brand: REAGENLLC
Rating: 4.1 (1 reviews)

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Model No. :	RND99005
Brand Name :	REAGEN

11yrs

Moorestown, New Jersey, United States

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Product description

Product Description

REA GEN ^™ Nitroimidazole ELISA Test Kit provides a competitive enzyme immunoassay for the quantitative analysis of Nitroimidazole in feed, meat (beef, chicken and pork), eggs, honey.

The unique features of the kit are:

High recovery (70% - 95%), and cost-effective extraction methods for various samples.
A quick ELISA assay (less than 1.5 hours regardless of number of samples).

High reproducibility.

Procedure Overview

The method is based on a competitive colorimetric ELISA assay. The nitroimidazole antibody has been coated in the plate wells. During the analysis, sample is added along with the nitroimidazole -horseradish peroxidase (HRP) conjugate. If the target is present in the sample, it will compete for the nitroimidazole antibody, thereby preventing the nitroimidazole- HRP from binding to the antibody attached to the well. The resulting color intensity, after addition of substrate((TMB), has an inverse relationship with the target concentration in the sample.

Kit Contents, Storage and Shelf Life

REAGEN^™Nitroimidazole ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C*. The shelf life is 12 months when the kit is properly stored.

Kit Contents	Amount	Storage
Nitroimidazole -coated Microtiter Plate	1 x 96-well plate (8 wells x 12 strips)	2-8°C
Nitroimidazole Standards: Negative control 0.5ng/mL 1.5 ng/mL 3 ng/mL 6 ng/mL 12 ng/mL 100ng/mL (spiking, optional)	0.8 mL 0.8 mL 0.8 mL 0.8 mL 0.8 mL 0.8 mL 0.8 mL	2-8°C
500x HRP-Conjugated	60 mL	2-8°C *
HRP Conjugate Diluent	15 mL	2-8°C
TMB Substrate	12mL	2-8°C
20X Wash Solution **	28 mL	2-8°C
Stop Buffer **	14 mL	2-8°C
Concentrate of Sample Extraction Buffer	30 g	2-8°C
Sample Dilution Buffer	20 mL X2	2-8°C

* If you are not planning to use the kit for over 1 months, store 500 x HRP-Conjugated at -20 ° C or in a freezer.

Sensitivity

Sample Type	Detection Limit (ng/g or ppb)
Meat/Liver/Kidney/Fish	0.5
Shrimp	0.5

Specificity

Analytes	Cross-Reactivity (%)
Nitroimidazole	100
Dimetridazole	100
Metronidazole	100
HydroxyNitroimidazole	32
Ronidazole	14
Ipronidazole	11
Nicarbazin	7
Halofuginone	3
Diclazuril	4
Robenidine	2