Medical Clinical Analysis Instrument Analyzer Gene Analyzer

Medical Clinical Analysis Instrument Analyzer Gene Analyzer

Genetic Analyzer Description

MLPA (Multiplex Ligation-dependent Probe Amplification) method detects multiple copy number changes of genes or loci sites. Nowadays, MLPA is used to check large numbers of hereditary disorders and tumor profiling. Since Dutch Scientist Jan Schouten first invented it, the MLPA method was first published in 2002 'Nucleic Acid Research'. The principle of the MLPA is to apply the specific probe design targeting a region of interest on each sample DNA. MLPA method consists of the following steps:

Denaturation > Hybridization Ligation > PCR MLPA Amplificons > Capillary Electrophoresis > Data Analysis

We use sample DNA put into the thermocycler to separate the DNA strands. Then, the targeted DNA strand will be hybridized by adding up the Left and Right probe oligonucleotides. The ligase enzyme will bind the probe oligos up immediately to adjacent target on the sample DNA. Therefore, the hybridized Left and Right probe oligo start catalyzing the creation of a covalent bond in between. Once the probe oligos are sealed, MLPA probes will be exponentially amplified using the single PCR primer pair.  

PCR reaction consists of the three steps: denaturation, primer annealing and primer elongation. Those steps of PCR amplification are repeated many times. The fluorescence labelled primer is incorporated into the size of the amplification products that will go through the capillary electrophoresis to pass a detector. The measured fluorescence is visualized as a peak pattern, the so-called electropherogram. The raw data from the capillary electrophoresis analyzer forms the input of the MLPA analysis.

DNA Analyzer Display