Human Interferon gamma inducible protein 16 (IFI16/p16) ELISA Kit

INTRODUCTION
For the quantitative determination of human IFI16/p16 concentrations.
Detection of species: Human
Detection medium: serum, plasma, tissue homogenates, cell culture supernates.

PRINCIPLE OF TEST
The kit is for the quantitative level of IFI16/p16 in the sample, adopt purified human
IFI16/p16 to coat microtiter plate, make solid-phase antibody, then add samples or
standards to wells with a labeled antibody specific to IFI16/p16, then add labeled HRP
to the well. After washing completely, add TMB substrate solution, TMB substrate
becomes blue color in wells that contains antibody - antigen - enzyme-antibody
complex, reaction is terminated by the addition of a stop solution and the color
change is measured at a wavelength of 450 nm. The concentration of IFI16/p16 in the
samples is then determined by comparing the O.D. of the samples to the standard
curve.