Product description
Test principle
The kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) to assay the level of Human Acylated Ghrelin(AG) in samples. Add Acylated Ghrelin(AG) to monoclonal antibody Enzyme well which is pre-coated with Human Acylated Ghrelin(AG)monoclonal antibody, incubation; then, add Acylated Ghrelin(AG)antibodies labeled with biotin, and combined with Streptavidin-HRP to form immune complex; then carry out incubation and washing again to remove the uncombined enzyme. Then add Chromogen Solution A, B, the color of the liquid changes into the blue, And at the effect of acid, the color finally becomes yellow. The chroma of color and the concenthumanion of the Human Substance Acylated Ghrelin(AG) of sample were positively correlated.